Journal: Data in Brief

Article Title: Supporting dataset of two integration-free induced pluripotent stem cell lines from related human donors

doi: 10.1016/j.dib.2021.107140

Figure Lengend Snippet: Reagents details.

Article Snippet: Differentiation Marker , FOXA2 REA APC , 1:11 , Miltenyi 130-107-827; AB_2651758.

Techniques: Immunocytochemistry, Marker, Control

Journal: Data in Brief

Article Title: Supporting dataset of two integration-free induced pluripotent stem cell lines from related human donors

doi: 10.1016/j.dib.2021.107140

Figure Lengend Snippet:

Article Snippet: Differentiation Marker , FOXA2 REA APC , 1:11 , Miltenyi 130-107-827; AB_2651758.

Techniques: Flow Cytometry, Software, Sterility, Membrane, Filtration, Expressing, In Vitro, Staining

Journal: Cell reports

Article Title: Runx2 regulates chromatin accessibility to direct the osteoblast program at neonatal stages

doi: 10.1016/j.celrep.2022.111315

Figure Lengend Snippet: (A) Signal intensities of chromatin accessibility and Runx2-DNA binding in Obs and Chos. The selected regions showing Ob- and Cho-distinct signatures and their shared signatures are shown. The number of selected genomic regions is indicated. Representative data obtained from at least biological duplicates are shown. (B) GREAT GO annotations of the selected regions, showing the top five enriched terms. (C and D) Enrichment of motifs in Ob- and Cho-distinct regions and their shared regions. Motifs with p < 1.0 × 10 −50 and target sequences with motifs >10% are shown (C). Also shown (D) is the Z score representing the relative gene expression of transcription factors in C2-Cho, C10-Cho, and S7-Ob. Genes were selected from the enriched motifs in (D). Average values obtained from biological triplicates were used. Transcription factors with fragments per kilobase of transcript per million mapped reads (FPKM) >5 are shown. (E) CisGenome browser screenshots of the flanking regions of Col10a1 and Col1a2 showing chromatin accessibility and the associations of transcription factors in Obs and Chos. Cell-type-distinct regions are highlighted in gray. (F) Venn diagram showing the overlap of ChIP-seq peaks. Top: Runx2, Foxa2, and Jun in Chos. Bottom: Runx2, Sp7, and Jun in Obs. ChIP-seq peaks distally located more than 500 bp from the nearest TSS were used. Peak numbers for each ChIP-seq peak are shown. (G) Boxplots showing the normalized reads of ATAC-seq at regions associated with the indicated number of transcription factors. Top: C10-Cho ATAC-seq at regions occupied by Runx2, Foxa2, and/or Jun in Chos. Bottom: S7-Ob ATAC-seq at the regions occupied by Runx2, Sp7, and/or Jun in Obs. The y axis value is log 2 (normalized reads + 1). The values were averaged from biological triplicates. *p < 0.0001 versus normalized reads with the one factor-associated site (Tukey-HSD analysis). See also and and .

Article Snippet: Mouse monoclonal anti-FOXA2 antibody , Merck , 17–10258; RRID:AB_11204693.

Techniques: Binding Assay, Expressing, ChIP-sequencing

Journal: Cell reports

Article Title: Runx2 regulates chromatin accessibility to direct the osteoblast program at neonatal stages

doi: 10.1016/j.celrep.2022.111315

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-FOXA2 antibody , Merck , 17–10258; RRID:AB_11204693.

Techniques: Western Blot, Immunohistochemistry, Recombinant, Multiplex Assay, Transgenic Assay, Software